Journal: Biology of Reproduction
Article Title: Establishment and characterization of a new immortalized human adenomyosis epithelial-like cell line, tAEC21 †
doi: 10.1093/biolre/ioaf255
Figure Lengend Snippet: The tAEC21 cell line is negative for estrogen and progesterone receptors. Early passage (p.5–10) tAEC21, 12Z, MCF-7 (positive ERα/PGR antibody control), THESC (negative PAX8 antibody control), TOV21G (negative ARID1A antibody control) cells were subcultured on 8-well chamber slides and processed for (A) estrogen (ESR1/ERα) and progesterone (PGR) receptors, (B) paired box (PAX)8, and AT-rich interaction domain 1A (ARID1A) immunofluorescence, nuclei-counterstained with DAPI (blue), and imaged using an AMG EVOS FL imaging system; scalebar: 200 μm.
Article Snippet: The TOV21G cell line was purchased from ATCC (#CRL-3577) and was maintained in a 1:1 ratio of Medium-199 (#M4530, Sigma-Aldrich, St. Louis, MO, USA) and MCBD-105 (#M6395, Sigma-Aldrich), supplemented with 15% FBS and 1% P/S [ ].
Techniques: Control, Immunofluorescence, Imaging